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    Recombinases are genetic recombination enzymes. DNA recombinases are widely used in multicellular organisms to manipulate the structure of genomes, and to control gene expression. These enzymes, derived from bacteria and fungi, catalyze directionally sensitive DNA exchange reactions between short (30–40 nucleotides) target site sequences that are specific to each recombinase. These reactions enable four basic functional modules, excision/insertion, inversion, translocation and cassette exchange, which have been used individually or combined in a wide range of configurations to control gene expression.[1][2][3][4][5]

    Types include:

    References[redigér | rediger kildetekst]

    1. ^ Nern, A; Pfeiffer, BD; Svoboda, K; Rubin, GM (aug. 23, 2011). "Multiple new site-specific recombinases for use in manipulating animal genomes". Proceedings of the National Academy of Sciences of the United States of America. 108 (34): 14198-203. doi:10.1073/pnas.1111704108. PMID 21831835.{{cite journal}}: CS1-vedligeholdelse: Dato automatisk oversat (link)
    2. ^ García-Otín, AL; Guillou, F (jan. 1, 2006). "Mammalian genome targeting using site-specific recombinases". Frontiers in bioscience : a journal and virtual library. 11: 1108-36. doi:10.2741/1867. PMID 16146801.{{cite journal}}: CS1-vedligeholdelse: Dato automatisk oversat (link)
    3. ^ Dymecki, SM; Kim, JC (apr. 5, 2007). "Molecular neuroanatomy's "Three Gs": a primer". Neuron. 54 (1): 17-34. doi:10.1016/j.neuron.2007.03.009. PMID 17408575.{{cite journal}}: CS1-vedligeholdelse: Dato automatisk oversat (link)
    4. ^ Luan, H; White, BH (okt. 2007). "Combinatorial methods for refined neuronal gene targeting". Current opinion in neurobiology. 17 (5): 572-80. doi:10.1016/j.conb.2007.10.001. PMID 18024005.{{cite journal}}: CS1-vedligeholdelse: Dato automatisk oversat (link)
    5. ^ Fenno, LE; Mattis, J; Ramakrishnan, C; Hyun, M; Lee, SY; He, M; Tucciarone, J; Selimbeyoglu, A; Berndt, A; Grosenick, L; Zalocusky, KA; Bernstein, H; Swanson, H; Perry, C; Diester, I; Boyce, FM; Bass, CE; Neve, R; Huang, ZJ; Deisseroth, K (jul. 2014). "Targeting cells with single vectors using multiple-feature Boolean logic". Nature methods. 11 (7): 763-72. doi:10.1038/nmeth.2996. PMID 24908100.{{cite journal}}: CS1-vedligeholdelse: Dato automatisk oversat (link)

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