Bruger:MikkelTNielsen/Via1-Cassette
The Via1-Cassette is a consumable used in automated image based cell counting. It functions as a single use pippette that aspire cells from suspension into the mixing channels and up to the measuring chamber where the automated cell counter can analyse the sample.
How it works[redigér | rediger kildetekst]
The cassette are preloaded in the mixing channels with immobilized acridine orange for a total stain and DAPI for non-viable, which stains all cell nuclei. These are used to define the total number of cells and dead cell populations.
It has a pre laser calibrated volume chamber which makes it possible to analyse a total volume of 1.4 µL sample stained with the fluorescent dyes that are mixed homogeneous into the measuring chamber via the mixing channels.
Users[redigér | rediger kildetekst]
People working with numerous procedures within biology and medicine who are counting cells, examples.: Lab Technicians, Researchers, Biologists etc.
- ^ Robbins, E.; Marcus, P. I. "J Cell Biol,". 1963. 18: p. 237-50.: https://www.ncbi.nlm.nih.gov/pubmed/?term=14079487. PMID 14079487.
- ^ Kubista, M.; Nordén, B. "Biochemistry". 1987. 26(14): p. 4545-53.: https://www.ncbi.nlm.nih.gov/pubmed/?term=3663606. PMID 3663606.
- ^ Shah, D. "Cytotechnology". 2006. 51(1): p. 39-44.
- ^ "Patent US8860938 B2". ChemoMetec A/S.
- ^ "ChemoMetec consumables". ChemoMetec A/S.